FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320363
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9400.046.7276151
TCTGTCG11600.044.6898278
TCGCTCT11450.038.9870534
GTCTTGC24100.035.853851
CGCTCTG12050.033.4607125
CTCGCTC12800.033.187633
TCTCGCT13100.032.426912
CTGTCGC18100.029.0387889
TTGCTCT30400.028.4211624
CGAATAC1050.0079751727.4286793
GTTTCAC33700.026.7063373
CTTGCTC29400.024.4898933
GTCTCAC24700.024.4880371
GGGTTTC38450.023.7836781
GTCTTAC9400.023.746821
GTTAGCC12750.023.717743
TATACTT7000.023.6572365
GTACGGC1851.9184584E-423.3534561
GTGTTAC10150.022.7015511
TCTTGCT34900.022.693012
Produced by FastQC (version 0.11.5)