FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095832
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10550.041.6088568
GTCTCGC9800.032.3106231
TCGCTCT9500.031.0583924
GTCTTGC19100.030.8957821
TACGACC1402.8908338E-530.8419594
GTACGTC1204.2397136E-429.9852353
CTGTCGC15600.029.061929
CTCGCTC10700.026.9026433
TCTCGCT11200.026.3441732
CGCTCTG10800.025.9872065
GTCTTAC5050.024.2256971
GTATTAG6250.024.1800961
TTGCTCT26900.023.809844
GTTCCGG3652.419256E-1023.6595861
TACACTG9150.023.5949425
CTACGAC1550.001888859923.2143783
GTGCTAA7750.023.2143761
GTATAGC3203.6670826E-822.488931
CTTGCTC25650.022.1645283
GTATAAA6850.022.0621321
Produced by FastQC (version 0.11.5)