FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548214
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11250.042.2383278
GTCTCGC11550.040.5608561
CTGTCGC14500.036.2468389
GTCTTGC24000.033.9346121
TCGCTCT13150.032.8504074
TACACCG2705.456968E-1231.9987325
TCTCGCT14250.029.8093452
GTGTTAG17450.027.2598951
TTGCTCT31950.025.463784
CGCTCTG17850.025.0074125
CTCGCTC15700.024.7633483
GTCCTAC7300.024.682711
TAATACT7300.024.6565594
TTACCGT2051.3628378E-524.5843934
TAACACG5150.023.7660484
GTCTCAC29700.023.296491
GTATTAT7750.023.249521
GTACAGG15450.022.8582671
GTAACAC8950.022.5242463
CTTGCTC32650.022.2717043
Produced by FastQC (version 0.11.5)