FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548214
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14200.041.5558551
TCGCTCT14450.039.8408664
TCTGTCG12250.038.771678
CTCGCTC16350.034.770913
GTCTTGC22450.034.298421
TCTCGCT17200.032.2158052
CGCTCTG16250.030.5564175
GTCTTAC10200.028.9261321
CTGTCGC17950.028.0633969
GTGTAAG12800.027.5481721
GTGTACG3700.027.2290821
GTATTAC5200.026.2940181
TTCACCG7950.026.2504985
GAGTGCA67850.025.878956
GTTTCAC34100.025.746143
AATACCG2001.1296468E-525.1869015
GGGTTTC36700.025.0986631
GTGTAAT9750.025.094641
GGAGTGC63500.025.045245
TATACTG7150.024.155275
Produced by FastQC (version 0.11.5)