FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2818133
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12800.040.478818
GTCTCGC12600.037.695891
GTCTTGC26800.034.9083371
TCGCTCT13950.034.5637744
CTGTCGC18950.030.759629
TCTCGCT16750.029.215592
CGCTCTG15300.027.7511625
CTCGCTC17250.027.1171993
TTGCTCT34900.027.0126044
GTACTAC4800.026.9868281
GTATAAC4900.024.9674071
CTTGCTC35250.024.9069923
GTCTCAC31450.024.4840771
TCTTGCT37800.023.6075252
GTGTAAC9200.023.4668081
GTATTAA9000.023.1886831
GTATAAA11850.023.0773471
GTCTTAC10950.023.0024721
GGGTTTC44300.022.4179531
GTTTCAC39400.022.2835433
Produced by FastQC (version 0.11.5)