FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751678
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12200.041.2904781
TCTGTCG12750.038.379798
TCGCTCT13600.034.923424
CTGTCGC16200.032.8715829
GTCTTGC23900.032.8201331
CGCTCTG15150.029.9253795
TCTCGCT15650.029.8889562
GTATTAG8750.028.7853641
CTCGCTC16600.027.3114153
TTACACT11350.026.6296354
GTCTTAC10300.026.5496081
TTGCTCT32900.026.2480534
TAATACT8150.025.6066134
TACACTG15750.025.130085
GTTACCG1450.00128128424.814973
GGAGTGC61500.023.519755
GAGTGCA65400.023.1075176
CGTTTCG1600.002272347222.4881579
GTGTTAG17950.022.450981
GTGTAAT11450.021.9975511
Produced by FastQC (version 0.11.5)