FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676922
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.041.4064678
GTACCGA1301.724304E-533.263931
GTCTTGC24050.032.6645851
CTGTCGC16350.031.7054629
GTCTCGC12450.029.5234131
TCGCTCT13000.028.2452964
CGTTTCG1358.4212935E-426.6658579
CTCGCTC14850.026.1810233
TCTCGCT15200.025.5781732
GTGTTAC11650.025.3643421
CGCTCTG14150.024.9321045
GTCTCAC31950.024.813471
TTGCTCT36050.024.3654174
GTACTAC5100.024.0239521
GGCTAGA16650.023.807521
CTATACT7400.023.3506414
GGGTAAG13500.022.956221
GTACCGT1902.2842993E-422.7595331
ACCATGT34800.022.757938
GTTTCAC40600.022.5214843
Produced by FastQC (version 0.11.5)