FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676922
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13150.041.043578
GTCTCGC11550.036.7603531
GTCTTGC25100.034.1179471
CCGATCG900.003763845431.9835829
TCGCTCT13400.030.0741184
CTGTCGC16700.029.3023249
GTATTAG8050.026.8185331
CGCTCTG14800.026.2567945
GTGTTAC10450.026.1683861
GTCTCAC33850.025.5112781
TCTCGCT16450.024.935532
CTCGCTC15950.024.8148483
TTGCTCT33700.024.557134
GTGTAAA17350.024.471591
GGTATAC6350.023.798811
GTATAGG7150.023.1489581
GTCTTAC11550.023.0531021
GTGTTAG18200.022.9332851
GTACTAT4850.022.2566171
GGAGTGC66700.022.2254755
Produced by FastQC (version 0.11.5)