FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584506
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12600.042.2637258
CTGTCGC15950.033.8381929
GTCTCGC11300.031.8418371
GTCTTGC22350.031.5540521
TCGCTCT12200.030.6725654
GTGTTAC12000.026.9859581
GTTTCAC37900.026.5824763
TAGTACT7450.026.0803874
ACCGTGT8650.025.7900478
GTCTCAC31050.024.5669271
CGCTCTG14700.024.4770585
CCGTGTT8250.024.4236559
GGGTTTC44650.023.692041
GTGTAAC7400.023.3392071
GTATAGC5250.023.302161
GTACTAT5900.023.1743831
GTATTAG8450.022.9939541
GTATAAG6900.022.9445841
GTGTTAG17300.022.8782671
CTCGCTC15600.022.6036243
Produced by FastQC (version 0.11.5)