FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584235
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11800.035.981218
GTCTCGC11750.031.8471971
GTCTTGC24950.031.4384921
CTGTCGC16600.026.010519
GTATAAG8350.025.8547611
TCGCTCT12500.025.330774
GTGTTAG19900.024.2285521
GTACGGA2101.6486225E-523.987471
GTCCTAC7550.023.8286131
TATACTG8950.023.317435
GTTAGCC13600.023.281963
TCTACGG2802.5202462E-723.1307752
TTGCTCT32200.022.7955474
GTATTAC6000.022.78811
GGGTTTC42000.022.7880971
CTCGCTC15650.022.5313643
GTATTAG8350.022.407461
GCGCGAT6450.022.3139299
GTACGGG2303.3304226E-521.9016061
TCTCGCT14800.021.8804652
Produced by FastQC (version 0.11.5)