FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624119
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12250.044.7149121
TCTGTCG10500.043.198718
TCGCTCT13100.040.120944
TCTCGCT14850.036.362552
CTCGCTC14650.033.910253
CTGTCGC14750.032.7041059
CGCTCTG15750.032.4561735
GTCTTGC25550.032.4400751
GTATATA10600.028.5573751
TTCACCG9150.027.5401615
GTCTTAC9550.027.1690221
GTATTAG8600.025.1418761
GTGTATA13200.025.116481
GGTATAC5800.024.8528881
GGAGTGC60600.024.2368985
GAGTGCA68500.024.0693536
ACCGTGT10200.023.9992838
GTATTAA7650.023.553391
GTCTCAC29000.023.3617171
TCACCGT10500.022.6278956
Produced by FastQC (version 0.11.5)