FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485624
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7750.050.167518
CTGTCGC10100.038.494879
TCGCTCT10450.034.450994
GTATTAA7250.033.7680781
GTCTCGC10300.032.157781
CTCGCTC11000.030.1101633
GTCTAGC8000.028.8021831
GTCTTGC20150.028.5877741
CGCTCTG11550.027.4284465
TCTCGCT11850.027.3438472
GTCTTAC7450.027.0624581
GTTCGGC1658.824888E-526.1838041
GTATTGA5800.026.0709441
GTGTTAC7750.025.0857721
GTGTTAA8300.024.2911
GTGTTAG13350.024.2715051
GTGTAGC8600.023.443641
GTACTAG4850.022.269731
GTATAAC3605.2550604E-922.0016691
GTCTCAC23800.021.7831651
Produced by FastQC (version 0.11.5)