FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485624
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9450.038.0972141
GTATTAG5650.031.8600581
TCGCTCT11600.031.036094
GTGTAAG9950.028.9462241
TCTGTCG10500.028.1140468
GTACGGC1050.00797576227.4283376
GTATTAA6850.027.3298851
CTCGCTC12600.026.8585343
CGCTCTG12700.026.0800915
GTCTTGC19100.026.0118221
GTATACG1400.001040762225.7156181
TCTCGCT13900.025.382612
GGAGTGC52100.024.876535
GAGTGCA55400.024.562976
GTGTTAC7650.024.4718571
GTTCCGA1801.5937179E-424.0012451
GTCTCAC23800.022.9927861
CTGTCGC14600.022.6847389
GTCTTAC7700.022.4427221
GGCTGGA74250.021.8193131
Produced by FastQC (version 0.11.5)