FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273536
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10650.034.4782378
GTACGAC850.002837312933.8824733
GTCTCGC10450.032.382891
CTGTCGC14200.031.9430719
CGTTTCG1402.8816012E-530.8565759
GTCTTGC20100.030.0896591
TCGCTCT11300.027.3983274
GTATTAC5050.027.0892051
GTGTAAC6950.025.8993721
GTGTTAG14550.025.7320481
CTCGCTC11850.025.5190773
GTATTAA6250.025.3440881
CGCTCTG12950.025.0193945
GTGTTAC8650.024.9711861
TAGCACG2401.6988142E-624.0000864
GTCCTAC7750.023.2258871
GTATATA8100.023.1111931
GAGTGCA56550.023.0446686
GTATAAG6300.022.8572241
GTCTCAC29400.022.7755891
Produced by FastQC (version 0.11.5)