FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160099
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9800.041.8772778
GTCTTGC21050.037.969131
TCGCTCT10150.036.1771054
CTGTCGC12300.032.7802739
GTCTCGC9550.032.4208871
GTATTAT5750.030.0540641
CGCTCTG11900.028.4367875
TAATACT6400.028.1248134
GTGTTAG13750.027.7544731
TTGCTCT29650.027.6827874
TCTCGCT12100.026.1834662
GTGTTAT7150.026.1834641
CTCGCTC12650.026.1816463
ACCCGCG1658.8293105E-526.1816467
CTTGCTC28200.026.0423813
GTATACG1701.08105305E-425.413361
GGGTTTC37050.025.07041
ACCGTGT6500.024.3690728
GTATTAG7400.024.3258531
GTATTAC5650.024.2139131
Produced by FastQC (version 0.11.5)