FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160099
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9450.040.383861
TCTGTCG9100.037.97468
GGTATAC4550.033.2331621
TCGCTCT9750.032.4946484
GTCTTGC20650.031.3824061
GTATTAG6900.031.30661
TCTCGCT11750.030.0276952
CTGTCGC12550.029.256339
CTCGCTC12000.027.0019443
GTATTGG6800.026.4724961
GTGTTAG13250.026.0848981
CGCTCTG11750.025.7380245
GTCTCAC24400.025.0837751
TAATACT5800.024.8293744
GGAGTGC51350.024.1185845
GTCTCGA20450.023.9391656
GTGTAAC6400.023.6267011
GTATAGA5800.023.5879061
GTGTTAC7950.023.5488661
TGCGGTA1851.9186336E-423.3530332
Produced by FastQC (version 0.11.5)