FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101459
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8700.045.5169948
GTCTTGC20450.040.8447381
CTGTCGC10550.034.80559
GTCTCGC9250.033.4732741
GTATACG1153.2925542E-431.3071581
TTGCTCT27850.028.439264
TTACACT9100.027.6934744
GTGTAAT8700.027.3127961
GTCTTAC8950.026.549871
TCTTGCT31000.026.0152382
CTCGATC8050.025.9377468
GTACTAA4450.025.8899631
CTTGCTC27200.025.4128363
TCTCGCT10900.025.103172
TCGATCT8350.025.0058529
GGTATAC4350.024.8298151
TCGCTCT10900.024.4413974
GTATAGA5900.024.408971
GTATTAG6800.024.3551271
GTCTCAC25850.024.2342821
Produced by FastQC (version 0.11.5)