FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866756
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7850.041.2736368
GTCTCGC9250.037.3679471
CTGTCGC9750.036.18449
GTCTTAC7550.032.428861
GTAGTCG900.003754220431.999816
TCGCTCT9050.031.8255714
GTCTTGC17050.028.7199861
TCTCGCT11150.027.1252752
CCGTGTT5900.025.6269669
CTCGCTC11650.025.340943
TAGTACT4001.8189894E-1225.2018764
GTGTAAT7350.024.4935871
GTACTAT4151.8189894E-1224.2929171
GGGTACG1500.001554818124.0037161
ACCGTGT6450.023.4417218
GTATTAT5850.023.3882331
GTATTAG6500.023.2651391
GGGTTTC31700.023.1707781
CGCTCTG11250.023.0398645
TTACTCT10150.022.7011954
Produced by FastQC (version 0.11.5)