FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866756
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC18750.034.9453961
TCGCTCT9550.032.4201474
GTCTCGC8450.032.3799931
TCTGTCG8450.032.3782588
GTGTTAC7800.029.5396441
GTATTAC4050.028.4455811
TACACTG11350.026.644245
GTATAAC3555.456968E-1226.367251
TAATACT5250.026.0581844
TTGCTCT25800.025.9545274
ATCGTAC1400.001040736425.7153153
GTGTAAA12050.025.096441
GTATAGG5250.024.68671
TACGTCT1751.3163697E-424.68674
CCGTGTT6150.024.5850329
CTTGCTC24150.024.4481833
GTCTCAC23900.024.402651
GGGTAAT6850.024.176151
TCTGTCA23300.024.1026768
ATACGGT1500.001556343223.9996746
Produced by FastQC (version 0.11.5)