FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924441
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8800.040.911411
TCGCTCT9400.030.6400324
GTATAGG3950.029.1662081
GTCTTGC20100.029.016571
TCTGTCG9000.027.9986768
GTCTTAC7000.027.7731
TCTCGCT11500.027.5493872
GTACGGG1607.158106E-527.001531
CTCGCTC11850.026.1280633
GTATTAC4450.025.8891071
TATACTG6200.025.5498335
GGGTTAC6400.024.7514021
GTATAAC3551.6916601E-1024.3394091
GTGTTAC8050.024.1504341
GTATTAT3902.1827873E-1124.001361
TTGCTCT24000.024.001364
TAATAGT3652.401066E-1023.6725734
CTGTTGC21600.023.6661649
GTATAAA6700.023.6431311
GAGTGCA48450.023.6273656
Produced by FastQC (version 0.11.5)