FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140407
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8800.037.6351058
GTCTTGC20850.032.853011
CGGTACT1453.6619713E-529.7921094
CTGTCGC12450.028.3364039
GTCTCGC10450.026.9095171
TCGCTCT12050.026.2895774
GTACCGT1400.001041232325.7134276
TAATACT7650.024.469774
GTGTAAG13150.024.1259561
GGGTTTC37500.024.0345691
GTGTTAC8450.023.8923531
GTTTCAC34100.023.879083
GTATAAT8500.023.751811
GTCTTAC8800.022.942091
GTGTATA12450.022.5867041
GTCTCAC26250.022.5238251
GTATAAG6750.022.4322641
GTCCAAG15550.022.2570951
TTGCTCT28800.022.2492544
CCGTGTT7450.022.2274469
Produced by FastQC (version 0.11.5)