FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140407
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10000.041.04038
GTCTCGC9250.035.02811
GTCTTGC23350.033.919671
TCGCTCT10250.032.3116764
TCTCGCT11100.027.8914432
GTATAAC4700.027.5753141
CTTGCTC28100.026.3910353
CTGTCGC15050.026.3124879
TTGCTCT29150.025.687414
GTACTAC3951.8189894E-1225.519771
CTCGCTC11900.024.8063263
CGCTCTG10900.024.4399765
GTATTAC6300.024.0007361
GGGTTTC39700.023.3961851
GTGTAAC6950.022.7920671
GTTTCAC37150.022.6752743
GGGCTAC6900.021.9137151
CAACGCT3355.925358E-821.4921914
CTCGATC6450.021.2094578
CCGTGTT7200.021.0001539
Produced by FastQC (version 0.11.5)