FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686942
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6700.045.1333548
GTCTCGC7350.043.157391
GCGCTAC1053.9768165E-641.1956941
TCGCTCT8650.039.1205444
TCTCGCT8650.036.645212
GTATAAT4900.035.3105931
CTCGCTC9150.033.068073
GTCTTGC16900.032.8468631
GTCTTAC6200.032.5578881
TTACACT7050.031.658894
CTGTCGC10600.031.2446089
CGCTCTG9100.029.2740925
GTATTAT6600.027.307751
GTGTAAA10450.026.215441
GTATAAA8000.026.1335181
GTGTATA9150.026.000561
TACACTA2808.8348315E-925.713735
GTCCGGG4051.8189894E-1224.9208531
GTCTCAC18600.024.806011
GTATTGC5250.024.7174151
Produced by FastQC (version 0.11.5)