FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686942
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7350.037.2240838
GTCTCGC7900.034.633561
TAATACT6400.031.4996574
GTCTTGC14900.031.4099861
TCGCTCT8800.031.090574
CGCTCTG8400.030.8568065
GGTATAC3251.8189894E-1228.8005391
TATACTG4900.027.9180625
TTCCGCG1050.007975141527.4282722
GTATTAG5800.026.0694541
CTCGCTC9400.026.0422693
GTCCGGA2259.679097E-725.6004811
TCTCGCT10250.024.5850962
ATAATAC5900.024.4065133
GTATAGT4450.024.2701191
GTATAAG4750.024.2530861
TATTAGG4750.024.2523672
GTGTAAG7450.024.1615281
GGCTAGA8650.024.139181
GTGTTAC7350.023.5106451
Produced by FastQC (version 0.11.5)