FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562479
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG657.5931055E-444.3067058
GTCTCGC7550.039.098471
TCGCTCT8600.035.99924
TCTGTCG7450.032.858338
CTCGCTC8300.031.2282243
TCTCGCT9300.029.4187012
GTACTAA3950.029.1639081
GTACTAG2802.8558134E-1028.2850881
GTATTAC3900.027.6916941
AATACCG1306.7532127E-427.691695
GGCGTAA1050.00797540327.4279611
GTCGTAA1050.00797540327.4279611
GTCTTGC15650.027.1431671
CGCTCTG9950.026.7732735
GTCTTAC5650.026.7604681
CCTACGT2259.681789E-725.5994323
CTGTCGC9950.024.6024689
CGGAACG1801.5941494E-423.9994684
TACACTG8750.023.039495
GTGTAAT6150.022.2434081
Produced by FastQC (version 0.11.5)