FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935295
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10050.038.6860928
TCGCTCT9800.035.999564
TATACCG850.00283742733.8819435
GTCTCGC8800.033.5450481
GTCTTGC17100.033.2627531
GCGTAGT1957.488779E-933.2303661
CGCTCTG10550.030.0280725
CTGTCGC13350.029.1232389
CGGTAAC1306.753403E-427.691971
TCTCGCT10550.027.2982462
GTTACAC4600.026.6083723
GTATTAA6300.026.2853951
GTCTCAC21000.025.7139721
TATACTG5900.025.6268065
GTTTCAC27600.025.5649053
CTCGCTC11750.025.1230983
TTGCTCT24300.024.5922934
GTCTAGC8200.024.5850661
CTTGCTC23250.023.844873
GTACAAT6350.023.8107341
Produced by FastQC (version 0.11.5)