FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602797
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6300.046.8588378
GTCTCGC7250.036.7909241
TCGCTCT7050.035.7437444
CGCTCTG7350.035.264385
CTGTCGC8250.034.0375949
CCGTTCG900.0037532932.001169
GTCTTGC14700.030.8958511
CTCGCTC8700.028.988273
GTCCGTA1000.006246070428.8361281
TCTCGCT8700.028.1600322
GTCTTAC6450.027.9419821
TAACGCT1050.0079756227.4278534
GTGTTAG11000.026.870031
GTGTTAC7500.025.9525151
TTGCTCT19600.025.7136144
TCAACCG1450.001277456624.8269355
GGTATAC3201.3260433E-924.7810481
GGTTTAC3851.8189894E-1124.3421881
ACTACGT1801.5935433E-424.0008688
TAATACT4550.023.7356414
Produced by FastQC (version 0.11.5)