FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602797
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8700.041.3800471
TCGCTCT8250.040.144924
GTCTTGC14000.038.057821
TCTGTCG9400.036.7654658
CTCGCTC9250.033.4698223
CGCTCTG9100.033.2303245
GTGTAAG7500.032.640581
TCTCGCT10150.031.9207532
TCGCACT1652.5202608E-630.5450444
GTATTAC4250.028.800511
TTGCTCT20800.028.7303834
CTGTCGC13450.027.3007479
GTATTAA5350.026.9163671
CTTGCTC19550.026.8845483
GTCCTAT3750.026.8804781
GTCTTAC6800.026.4710581
GTATAAG5000.025.9204621
GTTTCAC24050.024.8478983
GTATAAC2901.2825694E-824.8280281
CCGTCAA1450.001277361324.8272539
Produced by FastQC (version 0.11.5)