FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418797
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9800.037.4699631
TCGCTCT10350.034.7824254
GTCTTGC22550.032.248831
TCTGTCG10700.030.9531068
TAATACT8250.028.7998474
GTGTAAC8150.027.3869251
GGTATAC5650.026.7614731
GTATAAC4600.026.6091041
ATAATCG1701.0816058E-425.411634
TATACTG8250.025.3089565
GTGTTAG17050.024.4930461
GTATTAG9750.024.3696041
GTAATAC5650.024.212263
GGGTTTC43650.024.0828441
TTGCTCT31650.023.8861274
GTACTAG4850.023.7529431
TCTCGCT13350.023.730212
GTTTCAC38550.023.7197193
CTCGCTC12900.023.4417363
GTCTCAC29400.023.2656631
Produced by FastQC (version 0.11.5)