FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471793
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9850.048.2444841
TCTGTCG13200.039.8180588
TCGCTCT12800.037.1248864
CTATGCG2104.0745363E-1034.2856069
GTCTTGC21250.034.2217641
CGCTCTG13050.032.5516245
CTGTCGC18350.031.7819189
CTCGCTC15050.031.0962493
TCTCGCT14300.030.713192
GTGTAAC7950.028.0759531
GTCTTAC10950.026.3018231
CTATACG1400.001041093825.7142074
GTATTAG7400.025.2977311
TTGCTCT32200.025.04344
GTGTTAG18350.024.7197691
GTCTCAC28000.024.1718431
GTTTCAC38050.023.842243
GGGTAAC8550.023.5793511
GGGTTTC41400.023.4786641
GTACGGT2151.969609E-523.4422631
Produced by FastQC (version 0.11.5)