FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2496873
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9300.041.0319378
TCGCTCT11600.038.4816864
GTCTCGC10400.037.4413071
CGCTCTG12550.030.979225
CTGTCGC13850.030.6712429
GTCTTGC20050.030.2103331
CTCGCTC12900.028.4894283
TCTCGCT13450.027.8602052
GTATTAC5750.025.0814571
GTCTAAC5450.023.8158761
GTACTAT6200.023.2610281
TAATACT7200.022.9993614
GTATAGA7400.022.4123131
GTCTAGC10250.021.8086321
GTATAAG8150.021.2346061
GTGTAAA13800.020.9012131
GTTCTAG13600.020.678371
GTATAGG7700.020.6026251
CTTACCG2104.5231872E-420.571279
CTACCGT1750.00381058620.5708564
Produced by FastQC (version 0.11.5)