FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2496873
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11050.035.185151
GTCTTGC20800.033.9227181
TCGCTCT12000.031.7996644
TCTGTCG12600.027.4282828
TCTCGCT13400.027.4026952
CTCGCTC12900.027.3485493
CGCTCTG13550.027.0993445
GTATTAT9450.022.0950051
GTCTCAC26350.021.8593521
GTGTAAC7250.021.8480451
GTATTCG1650.002708764721.817951
GTATATA10750.021.4323311
GTATTGA7850.021.095321
TTGCTCT30900.020.9706544
GCGCTAG2755.538428E-620.9452341
CTGTCGC18400.020.738919
GTGTTAC9900.020.363421
GTCCAGA16550.020.0118751
GTACTGA8350.019.8321251
GTATAGG6200.019.7417261
Produced by FastQC (version 0.11.5)