FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4219227
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18200.038.3121388
GTCTCGC18600.034.4013021
GTCTTGC38900.032.8979031
CTGTCGC22050.032.600749
TCGCTCT20400.030.6566814
GTGTTAG28200.027.2792551
TCTCGCT22750.026.8618142
CGCTCTG23250.025.3528615
GTCTTAC16600.025.119891
GTATTAA12900.025.0796011
CTCGCTC23550.024.7267023
GTATTAT13300.023.2442041
TTGCTCT56300.022.3442464
GCGTTAC2603.4502445E-622.1214961
GTGTTAC14350.022.0444161
GTGTTAT18300.021.6077441
CTTGCTC51950.021.4495533
TCTTGCT59000.021.3247452
TCTGTCA51600.020.7573858
GTCTCAC49900.020.6031361
Produced by FastQC (version 0.11.5)