FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4219227
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC19400.038.6002351
TCGCTCT22700.034.2555164
TCTGTCG20350.033.2572068
GTCTTGC39350.032.0222321
TCTCGCT24450.030.6275882
CTCGCTC24950.030.0120323
TACGCGT1255.3697376E-428.8000094
CGCTCTG26550.027.6610245
GTATTAC9800.024.2463421
CTGTCGC29050.024.0407459
GTATTAA12950.023.3527411
GTGTTAG26000.023.2629241
GTCTTAC17050.023.2271881
TTGCTCT53450.022.7652074
GTATTAG12800.022.5013391
GTTTCAC66900.022.2780323
GTATAAA15500.021.8335571
GGGTTTC73500.021.6502691
GTCTCAC48350.021.2959941
CTTGCTC49700.021.295783
Produced by FastQC (version 0.11.5)