FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4144598
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC17500.036.1489751
TCTGTCG17200.035.1064158
TCGCTCT18400.031.6448354
GTCTTAC16800.030.3808881
CTGTCGC22400.029.8449299
GTCTTGC37350.029.8326951
TCTCGCT20550.029.0347212
GTCGTAC1555.8322614E-527.827291
CGCTCTG20700.026.045135
CTCGCTC22150.025.6382873
CCGTTCG2259.82287E-725.5589549
GTATTAT12050.024.4595341
GTATTAG13500.022.8973921
GTACTAT8500.022.8347471
GTATAAG12350.022.7012121
GTGTAGG18800.022.1779561
GTGTAAA23800.022.0494251
GTGTAAG20600.020.5890451
GTCTAGC16350.020.2251361
TCTGTCA53550.020.1357338
Produced by FastQC (version 0.11.5)