FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3975124
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14950.038.466198
GTCTTGC37400.033.061311
GTCTTAC16550.033.012531
CTGTCGC18750.032.20399
TCGCTCT17700.032.0836874
GTCTCGC17200.030.9290451
GTATTAC10150.028.3306921
GGTATAC7150.027.1469421
GTGTTAC16150.026.7080351
TTGCTCT52450.025.0804924
GTACTAC7200.023.9630431
CTCGCTC20700.023.962143
TCTCGCT20150.023.546812
GTATTAT12600.023.3924941
GTGTATA20800.023.1565931
GTTTCAC64150.022.6360953
CGCTCTG20350.022.6071595
GTGTAAT17000.022.4124931
GTATTAA11900.022.3520811
GTAATAC8800.022.0560593
Produced by FastQC (version 0.11.5)