FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3975124
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC18350.041.9864271
TCTGTCG19100.037.6955038
TCGCTCT20150.033.944664
GTCTTGC36150.033.2636071
CGCTCTG22450.029.8256245
CTGTCGC26400.029.4538969
CTCGCTC22800.029.3677773
TCTCGCT25150.028.057522
TTGCTCT52500.024.548034
GTATAAC6750.024.5349541
GTCTTAC15400.024.3132931
CTTGCTC49150.024.1703743
GTACTAG8050.024.1506611
GTGTTAG26650.024.0466161
GTGTTAC15450.023.768561
GTATTAA12450.023.7124081
TAATACT11500.023.1647074
GTGTTAA15250.022.6637921
GTATAAT12550.022.375981
TCTGTCA50600.022.054858
Produced by FastQC (version 0.11.5)