FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4446902
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT21650.033.5885624
TCTGTCG20350.032.903468
GTCTCGC19500.032.125271
CTCGCTC24300.028.1478543
GTCTTGC41350.027.8616331
GTCTTAC18400.025.827851
TCTCGCT25400.025.7970372
CTGTCGC28250.024.9764489
CGCTCTG24350.024.8375235
GTATTAG14100.023.4909651
GTATTAT13600.022.766261
GGTATAC9200.021.914541
GTTTCAC67450.021.6691593
GGGTTTC74850.021.2599661
GTGTTAG28900.021.1779161
CGTTTCG2455.4001684E-520.570989
GTATTAA14400.020.50141
GTATAAT12650.020.4915161
CTGTTGC47050.020.1993489
GGAGTGC100700.020.091155
Produced by FastQC (version 0.11.5)