FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3795446
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16350.040.9556121
TCGCTCT18350.036.0975044
TCTGTCG17200.033.487828
GTCTTGC36150.033.2626151
CTCGCTC21800.028.7334753
TCTCGCT23650.028.0094742
CGCTCTG20750.027.7585815
CTGTCGC22200.027.2427989
GTGTTAG24750.024.7281721
TTATACT13400.024.7160134
GGGTTTC64600.024.6324751
TTGCTCT52500.024.1367474
GTCTTAC16500.023.5644911
TTACACT19850.022.8510114
GTATATA16350.022.0191481
GTTTCAC60100.021.9231033
GTATTGC11500.021.913841
GTATTAG12600.021.7150751
CTGTTGC42450.021.7098929
TATACTG12650.021.6281035
Produced by FastQC (version 0.11.5)