FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3691456
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT19150.035.342034
GTCTCGC17000.034.7298771
TCTGTCG16700.033.197158
GTCTTGC36100.031.113991
CTCGCTC22250.028.7999943
CGCTCTG22650.027.9735055
TCTCGCT23350.025.9018462
CTGTCGC22900.025.1524939
GTCTTAC14650.025.065181
GTATTAG11700.023.3849281
GTATAAT11100.023.3516641
GTGTAAA23950.022.5472741
TATACCG1650.002708843721.8181785
GTATTAC8600.021.7677331
TTATACT13200.021.2727244
GTATAGG9900.021.091191
GTATAGA14150.020.8624691
GTGTACG4851.8189894E-1120.7837831
GTCTCAC44950.020.6632351
TACGACT2455.3989545E-520.5714244
Produced by FastQC (version 0.11.5)