FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4879053
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC19400.032.6625061
TCGCTCT20950.030.9308224
TCTGTCG21450.027.85968
GTCTTGC44600.027.1233311
CGCTCTG23200.025.4483035
GTATTAG16400.024.1483481
CTCGCTC24400.023.9014193
TCTCGCT26250.023.864842
GTATTAA14700.023.5121571
GTGTTAG31500.022.173271
GTGTTAC16950.022.090331
GTATAAC7350.021.552811
GTACGGG2704.722353E-621.3351061
CTGTCGC28550.021.1834779
GTCTTAC19050.021.1671121
GTATTGG14850.020.3653261
GTGTAAG22350.020.2969881
GTACTAT10700.020.1885931
GTGTTAA20000.020.1616741
GTCTCAC55050.020.1433621
Produced by FastQC (version 0.11.5)