FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482162
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10350.036.419841
TATCCGA850.002839044733.8783766
TCTGTCG11700.031.9962438
TCGCTCT11300.029.9433864
GTCTTGC22150.029.453971
GTATTAG8350.027.7804031
CTCGCTC12900.027.3456293
GGTATAC5350.027.0988751
GTGTTAC10550.026.7970641
GTATAAG7950.026.4427071
TCTCGCT13900.026.4136332
ACCGTGT6950.025.896248
GGAGTGC53900.023.641015
GTATACG1851.8335268E-423.5101051
GTGCTAC8350.023.4397161
GAGTGCA57450.023.3079686
CGCTCTG14350.023.0774295
GTCTCAC28750.022.6923641
GTACTAT5900.022.115441
GTATAGA8550.022.0435891
Produced by FastQC (version 0.11.5)