FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2877250
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12400.033.334361
TCTGTCG12850.032.4941568
TCGCTCT14450.029.402574
GTCTTGC28450.029.3126091
GTATTAC5950.028.0317211
TCTCGCT15900.027.6380582
GTATAAC5000.027.5564041
GTGTTAC12150.026.8580911
CTCGCTC15750.025.6142853
GTATATA12550.024.846411
GTATTAA9650.024.7985081
GTGTAAA17050.024.6684861
CGCTCTG15900.024.0037925
GTATAAG9450.023.7885931
GCGTAGT2802.34053E-723.3089871
TTGCTCT36700.022.9573064
GTATAGA9800.022.9390031
CTGTCGC18800.022.5938179
GTCTCAC32850.022.5166451
GTAGCGC2252.7960825E-522.4035386
Produced by FastQC (version 0.11.5)