FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2877250
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10450.043.0884551
TCGCTCT12750.032.1826554
GTCTTGC23850.031.6687181
TCTGTCG12550.030.4000458
TCTCGCT15150.027.0834712
GGTATAC5900.025.8495391
CTCGCTC16100.025.0391373
CGCTCTG14350.024.5811025
GTCTTAC11150.024.0997411
GTATTAG9950.024.0866491
GTCTCAC30850.023.7767091
GTGTTAC11500.023.366271
GTGTAAG16850.023.2744461
CGTAGGA3203.65726E-822.4953162
GTATATA14000.021.7874681
GTCTAGG14100.021.6329481
GGGTTTC46600.021.5069431
GCGTAGG3508.592724E-820.749971
GGAGTGC62450.020.7490025
CTGTCGC20600.020.6170659
Produced by FastQC (version 0.11.5)