FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408972
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10150.043.975888
TCGCTCT11150.041.9775924
GTCTCGC11250.041.907221
CTCGCTC11550.034.9193533
CGCTCTG12250.034.093455
TCTCGCT13000.033.7945482
GTCTTGC18500.033.3253861
CTGTCGC14900.029.9567229
GTCTCAC23150.027.8847661
GTACAAA7300.026.8268031
GTCTTAA7200.026.192011
TTACACT8250.026.1846244
TAACACT6300.025.1455524
TTTCGAC1450.001275260724.8348873
ACCGGTT1450.001278195824.8250928
GTTTCAC28800.024.5072043
TCACTCT26800.024.450384
GTCTAGC8950.024.312311
GGGTTTC31700.024.0247021
GTTTCGC4850.023.927991
Produced by FastQC (version 0.11.5)