FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408972
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11100.041.2256741
TCGCTCT12400.035.4124344
GTCTTAC6850.034.992391
TCTGTCG13550.034.532718
CTCGCTC13000.032.6705363
CGCTCTG13250.030.9681725
TCTCGCT14200.030.4165882
GTCTTGC16000.029.9622331
GTCTCAC23800.027.1620941
GTACTAA3005.7480065E-1026.6330951
TCACTCT26700.026.42184
GTATTAC3900.026.0743581
TCTGTCA22850.024.888438
GTCTAAC4550.023.945841
CTGTCGC18300.023.2090599
TTGCTCT22200.022.6982654
GTATAAA8050.022.5576741
GTCCAGA13900.022.4700411
CTGTTGC21750.021.8444589
ATCGAAC1650.002711318221.8143718
Produced by FastQC (version 0.11.5)