FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314972
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12950.043.9182178
GTCTCGC15000.038.6672131
TCGCTCT15500.037.6292764
TCTCGCT17150.032.3373152
CTCGCTC16200.032.0106773
CATACCG1504.6115376E-528.8026565
GTCTTGC25400.028.2582721
CGCTCTG17800.027.9126875
GTCTCAC30700.026.686041
CTGTCGC18950.026.2144269
GTATAAC4900.023.6738011
GTATTAA8350.022.5751671
GTCTTAC10650.022.4651031
GTATAGC6750.021.4817831
GGCTAGA17950.021.4069861
GGAGTGC70350.021.3921645
GTCTAGC10950.021.1875111
GTCCTAT7200.021.146131
GAGTGCA76600.021.056776
TCACTCT37500.020.9299324
Produced by FastQC (version 0.11.5)