FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314972
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14000.040.9829641
TCGCTCT15400.033.6583824
GTCTTGC26350.032.2485051
TCTGTCG16200.030.218228
CGCTCTG16350.029.0608065
CTCGCTC17900.027.3487453
TCTCGCT19850.026.8373492
GTCTTAC12000.026.6302811
GTATTAC6450.023.6463381
GTATAGG8400.023.3447281
TTGCTCT39300.023.2644424
GTATTAG9550.022.8150921
GTATAAG9000.022.595391
GTCTCAC33500.022.113611
CTGTCGC23300.021.9370069
CTTGCTC33800.021.7252623
GTACTAC4358.185452E-1121.7049311
GGCTGGA92150.021.5953161
TTACACT14050.021.5206034
TAATACT7700.021.5039674
Produced by FastQC (version 0.11.5)