FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2964846
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG1856.1532537E-627.2373459
GTCTAAG8700.026.7226371
GTCTCGC11000.026.418971
GTCTCAC30200.025.500431
TCTGTCG12750.025.4062618
GTCTTAC8650.024.3573741
TACCCGT2802.5122608E-723.1386265
GTCTTGC22350.023.079661
TCGCTCT13750.022.5122474
GTATAAG9000.021.795651
TACACTG14900.021.7409925
GGTATAC4801.2732926E-1121.1902161
GTATTAG9300.021.0925641
TCACTCT33500.021.0588344
GTACATA7100.020.4653991
GTTACAC6450.020.089353
GTATTGA9450.019.9889561
TTACACT11950.019.879214
TTGCTCT31650.019.7878514
TAATACT8450.019.594054
Produced by FastQC (version 0.11.5)