FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2773024
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.037.4234738
GTCTCGC10750.035.7609861
GTCTTGC26000.030.4085161
TCGCTCT12000.028.802244
CTCGCTC12650.026.760793
CTGTCGC16450.026.259189
GTGTTAG22450.025.847331
GTATACG2259.0799585E-725.7898921
GTATTAG9450.025.329361
TCTCGCT13400.024.7254752
GTGTTAC12150.023.879531
GTGTAAC9600.023.422461
ATACGAC1550.001879603623.2343183
GTCTCAC35100.022.7314761
CGCTCTG15600.022.6171445
GTGTAAG15200.022.4282971
GTATTAA9100.022.3181741
TTGCTCT34450.022.1555674
TAACACT14350.022.0783714
GACCCGT3305.0502422E-821.8198786
Produced by FastQC (version 0.11.5)